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PXD026060-1

PXD026060 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly
DescriptionCells respond to stress by blocking translation, rewiring metabolism, and forming transient mRNP assemblies called stress granules (SGs). After stress release, re-establishing homeostasis requires energy-consuming processes. However, the molecular mechanisms whereby cells restore energy production to disassemble SGs and reinitiate growth after stress remain poorly understood. Here we show that, upon stress, the ATP-producing enzyme Cdc19 forms inactive amyloids, and that their rapid re-solubilization is essential to restore energy production and SG disassembly. Cdc19 re-solubilization is initiated by the glycolytic metabolite fructose-1,6-bisphosphate (FBP), which directly binds Cdc19 amyloids and facilitates conformational changes that allow Hsp104 and Ssa2 chaperone recruitment. FBP then promotes Cdc19 tetramerization and activity, which together with trehalose breakdown boosts glycolysis to further enhance SG disassembly. Together, these results provide a molecular mechanism protecting cells during stress by directly coupling metabolism and ATP production with SG dynamics via regulation of Cdc19 amyloids.
HostingRepositoryPRIDE
AnnounceDate2021-08-17
AnnouncementXMLSubmission_2021-08-17_02:02:46.269.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIlaria Piazza
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue
InstrumentQ TRAP
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-17 10:42:28ID requested
12021-08-17 02:02:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cdc19, aggregation, SRM
Contact List
Paola Picotti
contact affiliationETH Zurich
contact emailpaola.picotti@imsb.biol.ethz.ch
lab head
Ilaria Piazza
contact affiliationETH Zurich
contact emaililaria.piazza@bc.biol.ethz.ch
dataset submitter
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Dataset FTP location
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