PXD026017-1

PXD026017 is an original dataset announced via ProteomeXchange.

Title	Identification of MukBHN conformational changes upon AcpP binding using cross-linking mass spectrometry
Description	Structural Maintenance of Chromosomes (SMC) complexes contribute ubiquitously to chromosome organization-segregation. SMC proteins have a conserved architecture, with a dimerization hinge and an ATPase head domain separated by a long antiparallel intramolecular coiled-coil. Dimeric SMC proteins interact with essential accessory proteins, kleisins that bridge the two subunits of an SMC dimer, and HAWK/KITE accessory proteins that interact with kleisins. The ATPase activity of the Escherichia coli SMC protein, MukB, is essential for in vivo function and is regulated by interactions with its dimeric kleisin, MukF, and KITE, MukE. Here we demonstrate that, in addition, MukB interacts with Acyl Carrier Protein (AcpP) that has essential functions in fatty acid synthesis. We characterize the AcpP interaction site at the joint of the MukB coiled-coil and show that the interaction is essential for MukB ATPase and for MukBEF function in vivo. Therefore, AcpP is an essential co-factor for MukBEF action in chromosome organization-segregation.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_09:00:56.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marjorie Fournier
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-14 02:44:49	ID requested
⏵ 1	2021-11-25 09:00:56	announced
2	2023-04-11 06:19:38	announced	2023-04-11: Updated project metadata.

Dataset with its publication pending

submitter keyword: Structural maintenance of chromosome, MukB, AcpP, cross-linking mass spectrometry

David Sherratt
contact affiliation	Department of Biochemistry, University of Oxford
contact email	david.sherratt@bioch.ox.ac.uk
lab head
Marjorie Fournier
contact affiliation	University of Oxford
contact email	marjorie.fournier@bioch.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026017
     1. Label: PRIDE project
     2. Name: Identification of MukBHN conformational changes upon AcpP binding using cross-linking mass spectrometry