PXD025980 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximity-dependent biotinylation detects association between stress granule associated proteins and SARS coronavirus nonstructural protein 1 |
Description | Severe acute respiratory syndrome coronavirus (SARS-CoV and SARS-CoV-2) nonstructural protein 1 (nsp1) is a critical viral protein that suppresses host gene expression by blocking the assembly of the ribosome on host messenger RNAs (mRNA). Using proximity-dependent biotinylation assay followed by proteomic analyses of biotinylated proteins, we captured multiple dynamic interactions of nsp1 with host cell proteins. In addition to ribosomal proteins, we have identified several mRNA processing proteins, including splicing factors and transcription termination proteins, exosomes- and stress granule (SG) associated proteins. The interactions with transcription termination factors are primarily governed by the C-terminal region of nsp1 and are disrupted by the mutation of K164 and H165 amino acids. We further show that nsp1 associates with SG protein G3BP and colocalizes with G3BP in SGs under sodium arsenite-induced stress for a short period of time. However, the presence of nsp1 disrupts the maturation of SGs over a long period. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_03:31:11.791.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jennifer Bethard |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-12 06:58:16 | ID requested | |
⏵ 1 | 2022-02-17 03:31:12 | announced | |
Publication List
Gerassimovich YA, Miladinovski-Bangall SJ, Bridges KM, Boateng L, Ball LE, Valafar H, Nag A, Proximity-dependent biotinylation detects associations between SARS coronavirus nonstructural protein 1 and stress granule-associated proteins. J Biol Chem, 297(6):101399(2021) [pubmed] |
Keyword List
ProteomeXchange project tag: Covid-19 |
submitter keyword: SARS-CoV, SARS-CoV-2, nsp1, stress granule, BioID2 |
Contact List
Anita Nag |
contact affiliation | 1Natural Sciences and Engineering, University of South Carolina Upstate, Spartanburg, SC 29303 |
contact email | ANITAN@USCUPSTATE.EDU |
lab head | |
Jennifer Bethard |
contact affiliation | Medical University of SC |
contact email | bethard@musc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025980
- Label: PRIDE project
- Name: Proximity-dependent biotinylation detects association between stress granule associated proteins and SARS coronavirus nonstructural protein 1