PXD025912-2

PXD025912 is an original dataset announced via ProteomeXchange.

Title	Acetylation level of Pannexin 1 lysine residues following alpha 1 adrenoceptor activation
Description	Pannexin 1 (Panx1) channels can be activated by alpha 1 adrenoceptor-induced pathways for the sympathetic regulation of blood pressure; however, the molecular mechanisms for this form of Panx1 channel activation remain elusive. To identify potential acetyl-lysine residue(s) of Panx1 channels that might be involved in this receptor-mediated Panx1 channel activation, we performed mass-spectrometry on Strep-tagged Panx1 proteins precipitated from the whole cell lysate of HEK293T cells after stable isotope labeling by amino acids in cell culture (SILAC). Those HEK293T cells were transiently transfected with alpha1D adrenoceptors and human Panx1-Strep, with or without phenylephrine stimulation. Equal amounts of light-labeled (control) and heavy-labeled (phenylephrine stimulated) cell lysates were pooled, and Panx1 proteins were precipitated by Strep-Tactin beads, followed by LC-MS-MS analysis. From three biological replicates, we identified a consistent, albeit modest, reduction of acetylation level at K140, following phenylephrine stimulation. Whereas the acetylation level of other lysine residues (K321, K374, K381, K409) were variable among three replicates or were unaffected by phenylephrine treatment. These results implicate Panx1 K140 as a potential regulatory site for receptor-mediated channel activation via an acetylation-deacetylation mechanism.
HostingRepository	PRIDE
AnnounceDate	2021-07-16
AnnouncementXML	Submission_2021-08-26_06:19:37.660.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu-Hsin Chiu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-10 02:01:09	ID requested
1	2021-07-16 08:46:09	announced
⏵ 2	2021-08-26 06:19:38	announced	2021-08-26: Updated publication reference for PubMed record(s): 34301959.

Chiu YH, Medina CB, Doyle CA, Zhou M, Narahari AK, Sandilos JK, Gonye EC, Gao HY, Guo SY, Parlak M, Lorenz UM, Conrads TP, Desai BN, Ravichandran KS, Bayliss DA, Deacetylation as a receptor-regulated direct activation switch for pannexin channels. Nat Commun, 12(1):4482(2021) [pubmed]

submitter keyword: Pannexin, Panx1, phenylephrine, alpha 1 adrenoceptor, SILAC, acetylation

Yu-Hsin Chiu
contact affiliation	National Tsing Hua University
contact email	yhchiu@life.nthu.edu.tw
lab head
Yu-Hsin Chiu
contact affiliation	National Tsing Hua University
contact email	yhchiu@life.nthu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025912
     1. Label: PRIDE project
     2. Name: Acetylation level of Pannexin 1 lysine residues following alpha 1 adrenoceptor activation