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PXD025912

PXD025912 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAcetylation level of Pannexin 1 lysine residues following alpha 1 adrenoceptor activation
DescriptionPannexin 1 (Panx1) channels can be activated by alpha 1 adrenoceptor-induced pathways for the sympathetic regulation of blood pressure; however, the molecular mechanisms for this form of Panx1 channel activation remain elusive. To identify potential acetyl-lysine residue(s) of Panx1 channels that might be involved in this receptor-mediated Panx1 channel activation, we performed mass-spectrometry on Strep-tagged Panx1 proteins precipitated from the whole cell lysate of HEK293T cells after stable isotope labeling by amino acids in cell culture (SILAC). Those HEK293T cells were transiently transfected with alpha1D adrenoceptors and human Panx1-Strep, with or without phenylephrine stimulation. Equal amounts of light-labeled (control) and heavy-labeled (phenylephrine stimulated) cell lysates were pooled, and Panx1 proteins were precipitated by Strep-Tactin beads, followed by LC-MS-MS analysis. From three biological replicates, we identified a consistent, albeit modest, reduction of acetylation level at K140, following phenylephrine stimulation. Whereas the acetylation level of other lysine residues (K321, K374, K381, K409) were variable among three replicates or were unaffected by phenylephrine treatment. These results implicate Panx1 K140 as a potential regulatory site for receptor-mediated channel activation via an acetylation-deacetylation mechanism.
HostingRepositoryPRIDE
AnnounceDate2021-07-16
AnnouncementXMLSubmission_2021-08-26_06:19:37.660.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYu-Hsin Chiu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-10 02:01:09ID requested
12021-07-16 08:46:09announced
22021-08-26 06:19:38announced2021-08-26: Updated publication reference for PubMed record(s): 34301959.
Publication List
Chiu YH, Medina CB, Doyle CA, Zhou M, Narahari AK, Sandilos JK, Gonye EC, Gao HY, Guo SY, Parlak M, Lorenz UM, Conrads TP, Desai BN, Ravichandran KS, Bayliss DA, Deacetylation as a receptor-regulated direct activation switch for pannexin channels. Nat Commun, 12(1):4482(2021) [pubmed]
Keyword List
submitter keyword: Pannexin, Panx1, phenylephrine, alpha 1 adrenoceptor, SILAC, acetylation
Contact List
Yu-Hsin Chiu
contact affiliationNational Tsing Hua University
contact emailyhchiu@life.nthu.edu.tw
lab head
Yu-Hsin Chiu
contact affiliationNational Tsing Hua University
contact emailyhchiu@life.nthu.edu.tw
dataset submitter
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