PXD025911-1

PXD025911 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of laser-microdissected murine fetal glomeruli
Description	Alteration of fetal kidney morphogenesis in diabetic pregnancies is poorly described, especially changes in the extracellular matrix (ECM) and glomerular basement membrane (GBM) during glomerulogenesis. Addressing the ECM proteome, or matrisome, in mouse fetal glomeruli in a healthy and diabetic environment will improve understanding about the association between ECM and GBM changes in the glomerulus as potential reprogramming mechanisms for glomerular dysfunction in infants of mothers with diabetes. This project aimed to define the matrisome and BM composition in the maturing murine fetal glomeruli collected from normal and diabetic mothers. For this, we used laser microdissection microscopy to isolate maturing glomeruli from cryosections, and analyse by label-free tandem mass spectrometry to test the hypothesis that maternal diabetes influences ECM and GBM composition, assembly and function.
HostingRepository	PRIDE
AnnounceDate	2021-10-13
AnnouncementXML	Submission_2021-10-13_00:06:53.051.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louise Hopkinson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-10 01:31:43	ID requested
⏵ 1	2021-10-13 00:06:53	announced

Dataset with its publication pending

submitter keyword: Glomerulus, extracellular matrix, laser microdissection microscopy, tandem mass spectrometry, glomerular basement membrane, mouse

Rachel Lennon
contact affiliation	Wellcome Centre for Cell-matrix biology, University of Manchester, UK.
contact email	rachel.lennon@manchester.ac.uk
lab head
Louise Hopkinson
contact affiliation	University of Manchester
contact email	louise.hopkinson@postgrad.manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD025911

PRIDE project URI

• PRIDE
  1. PXD025911
     1. Label: PRIDE project
     2. Name: Proteomic analysis of laser-microdissected murine fetal glomeruli