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PXD025911

PXD025911 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of laser-microdissected murine fetal glomeruli
DescriptionAlteration of fetal kidney morphogenesis in diabetic pregnancies is poorly described, especially changes in the extracellular matrix (ECM) and glomerular basement membrane (GBM) during glomerulogenesis. Addressing the ECM proteome, or matrisome, in mouse fetal glomeruli in a healthy and diabetic environment will improve understanding about the association between ECM and GBM changes in the glomerulus as potential reprogramming mechanisms for glomerular dysfunction in infants of mothers with diabetes. This project aimed to define the matrisome and BM composition in the maturing murine fetal glomeruli collected from normal and diabetic mothers. For this, we used laser microdissection microscopy to isolate maturing glomeruli from cryosections, and analyse by label-free tandem mass spectrometry to test the hypothesis that maternal diabetes influences ECM and GBM composition, assembly and function.
HostingRepositoryPRIDE
AnnounceDate2021-10-13
AnnouncementXMLSubmission_2021-10-13_00:06:53.051.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLouise Hopkinson
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-10 01:31:43ID requested
12021-10-13 00:06:53announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Glomerulus, extracellular matrix, laser microdissection microscopy, tandem mass spectrometry, glomerular basement membrane, mouse
Contact List
Rachel Lennon
contact affiliationWellcome Centre for Cell-matrix biology, University of Manchester, UK.
contact emailrachel.lennon@manchester.ac.uk
lab head
Louise Hopkinson
contact affiliationUniversity of Manchester
contact emaillouise.hopkinson@postgrad.manchester.ac.uk
dataset submitter
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Dataset FTP location
PRIDE project URI
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