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PXD025621-1

PXD025621 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSuccinylation profiles of brain injury after intracerebral hemorrhage
DescriptionProtein posttranslational modifications (PTMs) has been shown to regulate biological processes of human diseases via expanding the genetic code and for regulating cellular pathophysiology, however, the system-wide changes at the PTMs levels in ICH brain remains little known. Given that succinylation is one of the important PTMs in regulating many biological processes. Therefore, in this study, we used a high-resolution mass spectrometry-based, quantitative succinyllysine proteomics approach to firstly investigated the ICH-associated brain protein succinyllysine modifications. We totally identified the concentration of approximately 6000 succinylation events and quantified approximately 3500 sites. Among them, 25 succinyllysine sites on 23 proteins were increased, while 13 succinyllysine sites on 12 proteins were downregulated after ICH. Additionally, the subcellular localization analysis of these significantly changed succinylproteins showed that 58.3% hyposuccinylated proteins were located in the mitochondria, while the percentage of succinylproteins located in mitochondria was decreased to about 35% in ICH brains with concomitant increasing in the percentage of cytoplasm to 30.4%. Further bioinformatic analysis showed that the succinylated proteins were mostly located in mitochondria and synapse-related subcellular spaces, and participate in many pathophysiological processes, such as metabolism, cytoskeleton organization, synapse working and ferroptosis, etc.. Moreover, we performed a combination analysis of our succinylproteomics data with previously published transcriptome data and found that most of the differentially succinylated proteins were distributed into neurons, endothelial cells and astrocytes. In conclusion, our analyses uncover a number of succinylation-affected processes and pathways in ICH brains and provide new insights for understanding ICH pathophysiological processes.
HostingRepositoryPRIDE
AnnounceDate2021-10-29
AnnouncementXMLSubmission_2021-10-29_05:20:12.089.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiao-Yi Xiong
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListsuccinylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-25 22:18:45ID requested
12021-10-29 05:20:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Intracerebral hemorrhage
brain injury
succinylation
Contact List
Xiaoyi Xiong
contact affiliationAcupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine
contact emailxiongxy_89@163.com
lab head
Xiao-Yi Xiong
contact affiliationAcupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine
contact emailxiongxy_89@163.com
dataset submitter
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