PXD025621

PXD025621 is an original dataset announced via ProteomeXchange.

Title	Succinylation profiles of brain injury after intracerebral hemorrhage
Description	Protein posttranslational modifications (PTMs) has been shown to regulate biological processes of human diseases via expanding the genetic code and for regulating cellular pathophysiology, however, the system-wide changes at the PTMs levels in ICH brain remains little known. Given that succinylation is one of the important PTMs in regulating many biological processes. Therefore, in this study, we used a high-resolution mass spectrometry-based, quantitative succinyllysine proteomics approach to firstly investigated the ICH-associated brain protein succinyllysine modifications. We totally identified the concentration of approximately 6000 succinylation events and quantified approximately 3500 sites. Among them, 25 succinyllysine sites on 23 proteins were increased, while 13 succinyllysine sites on 12 proteins were downregulated after ICH. Additionally, the subcellular localization analysis of these significantly changed succinylproteins showed that 58.3% hyposuccinylated proteins were located in the mitochondria, while the percentage of succinylproteins located in mitochondria was decreased to about 35% in ICH brains with concomitant increasing in the percentage of cytoplasm to 30.4%. Further bioinformatic analysis showed that the succinylated proteins were mostly located in mitochondria and synapse-related subcellular spaces, and participate in many pathophysiological processes, such as metabolism, cytoskeleton organization, synapse working and ferroptosis, etc.. Moreover, we performed a combination analysis of our succinylproteomics data with previously published transcriptome data and found that most of the differentially succinylated proteins were distributed into neurons, endothelial cells and astrocytes. In conclusion, our analyses uncover a number of succinylation-affected processes and pathways in ICH brains and provide new insights for understanding ICH pathophysiological processes.
HostingRepository	PRIDE
AnnounceDate	2021-10-29
AnnouncementXML	Submission_2021-10-29_05:20:12.089.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiao-Yi Xiong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	succinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-25 22:18:45	ID requested
⏵ 1	2021-10-29 05:20:12	announced

Dataset with its publication pending

submitter keyword: Intracerebral hemorrhage

brain injury

succinylation

Xiaoyi Xiong
contact affiliation	Acupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine
contact email	xiongxy_89@163.com
lab head
Xiao-Yi Xiong
contact affiliation	Acupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine
contact email	xiongxy_89@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025621
     1. Label: PRIDE project
     2. Name: Succinylation profiles of brain injury after intracerebral hemorrhage