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PXD025618-1

PXD025618 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDisruption of nucleocytoplasmic trafficking as a cellular senescence driver
DescriptionSenescent cells exhibit a reduced response to intrinsic and extrinsic stimuli. This reduction could be explained by disrupted nuclear transmission of signals. However, this hypothesis required more evidence to complete as a new modality of cellular senescence. Proteomic analysis of the cytoplasmic and nuclear fractions from young and senescent cells revealed disruption of nucleocytoplasmic trafficking (NCT) as an essential feature of replicative senescence (RS) at the global level. Blocking NCT either chemically or genetically induced RS-like senescence phenotypes, named as nuclear barrier-induced senescence (NBIS). Transcriptomic analysis revealed that NBIS had the most similar gene expression pattern to RS, compared with other stress-induced types of cellular senescence. Core proteomic and transcriptomic shared patterns between RS and NBIS included upregulation of endocytosis-lysosome network and downregulation of NCT in senescent cells, which were also conserved in yeast aging model. These results implicate an aging-dependent coordinated reduction in the transmission of extrinsic signals to the nucleus and in the nucleus-to-cytoplasm supply of proteins/RNAs. We further showed that the aging-associated decrease in Sp1 transcription factor expression was responsible for downregulation of NCT. Our results suggest that NBIS is a modality of cellular senescence that can represent the nature of physiological aging in eukaryotes.
HostingRepositoryPRIDE
AnnounceDate2021-06-18
AnnouncementXMLSubmission_2021-06-17_19:43:30.243.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD025618
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJi-Hwan Park
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcarbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-25 22:11:10ID requested
12021-06-17 19:43:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cellular senescence, replicative senescence, nucleocytoplasmic trafficking, nuclear barrier, eukaryotic aging
Contact List
Daehee Hwang
contact affiliationDepartment of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea
contact emaildaehee@snu.ac.kr
lab head
Ji-Hwan Park
contact affiliationKorea Bioinformation Center (KOBIC), Korea Research Institute of Bioscience and Biotechnology (KRIBB)
contact emailkp706@postech.ac.kr
dataset submitter
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