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PXD025598-1

PXD025598 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA three-organelle complex made by wrappER contacts with peroxisomes and mitochondria responds to liver lipid flux changes
DescriptionHepatic lipid homeostasis depends on intracellular pathways that respire fatty acid in peroxisomes and mitochondria, and on systemic pathways that secrete fatty acid into the bloodstream, either free or condensed in very-low-density lipoprotein (VLDL) triglycerides. These systemic and intracellular pathways are interdependent, but it is unclear whether and how they integrate into a single cellular circuit. Here, we report that mouse liver wrappER, a distinct endoplasmic reticulum (ER) compartment with apparent fatty acid- and VLDL secretion functions, connects peroxisomes and mitochondria. Correlative light electron microscopy, quantitative serial section electron tomography and three-dimensional organelle reconstruction analysis show that the number of peroxisome-wrappER-mitochondria complexes changes throughout fasting-to-feeding transitions and doubles when VLDL synthesis stops following acute genetic ablation of Mttp in the liver. Quantitative proteomic analysis of peroxisome-wrappER-mitochondria complex-enriched fractions indicates that the loss of Mttp upregulates global fatty acid ?-oxidation, thereby integrating the dynamics of this three-organelle association into hepatic fatty acid flux responses. Therefore, liver lipid homeostasis occurs through the convergence of systemic and intracellular fatty acid-elimination pathways in the peroxisome-wrappER-mitochondria complex.
HostingRepositoryMassIVE
AnnounceDate2021-10-13
AnnouncementXMLSubmission_2021-10-13_13:03:12.627.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuca Pellegrini
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-23 06:52:20ID requested
12021-10-13 13:03:13announced
Publication List
no publication
Keyword List
submitter keyword: WrappER, mitochondria, peroxisome, inter-organelle contact, fatty acid, beta-oxidation, very-low-density lipoprotein, Mttp
Contact List
Luca Pellegrini
contact affiliationLaval University
contact emailluca.pellegrini@fmed.ulaval.ca
lab head
Luca Pellegrini
contact affiliationLaval university
contact emailluca.pellegrini@fmed.ulaval.ca
dataset submitter
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Dataset FTP location