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PXD025562-1

PXD025562 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn-cell structures of a conserved supramolecular array at the mitochondria-cytoskeleton interface in mammalian sperm
DescriptionMitochondria-cytoskeleton interactions modulate cellular physiology by regulating mitochondrial transport, positioning, and immobilization. However, there is very little structural information defining mitochondria-cytoskeleton interfaces in any cell type. Here, we use cryo-focused ion beam milling-enabled cryo-electron tomography to image mammalian sperm, where mitochondria wrap around the ciliary cytoskeleton. We find that mitochondria are tethered to their neighbors through inter-mitochondrial linkers and are anchored to the cytoskeleton through ordered arrays on the outer mitochondrial membrane. We use subtomogram averaging to resolve in-cell structures of these arrays from three mammalian species, revealing they are conserved across species despite variations in mitochondrial dimensions and cristae organization. We find that the arrays consist of boat-shaped particles anchored on a network of membrane pores whose arrangement and dimensions are consistent with voltage dependent anion channels. Proteomics and in-cell cross-linking mass spectrometry suggest that the conserved arrays are composed of glycerol kinase-like proteins. Ordered supramolecular assemblies may serve to stabilize similar contact sites in other cell types where mitochondria need to be immobilized in specific subcellular environments, such as in muscles and neurons.
HostingRepositoryPRIDE
AnnounceDate2021-10-29
AnnouncementXMLSubmission_2021-10-29_01:51:47.562.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRiccardo Zenezini Chiozzi
SpeciesList scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-22 06:42:36ID requested
12021-10-29 01:51:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proteomics, crosslinking, boar sperm, mitochondria, cryo-electron tomography
Contact List
Albert Heck
contact affiliationBiomolecular Mass Spectrometry & Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, The Netherlands
contact emaila.j.r.heck@uu.nl
lab head
Riccardo Zenezini Chiozzi
contact affiliationUtrecht university
contact emailr.zenezinichiozzi@uu.nl
dataset submitter
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Dataset FTP location
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