PXD025562

PXD025562 is an original dataset announced via ProteomeXchange.

Title	In-cell structures of a conserved supramolecular array at the mitochondria-cytoskeleton interface in mammalian sperm
Description	Mitochondria-cytoskeleton interactions modulate cellular physiology by regulating mitochondrial transport, positioning, and immobilization. However, there is very little structural information defining mitochondria-cytoskeleton interfaces in any cell type. Here, we use cryo-focused ion beam milling-enabled cryo-electron tomography to image mammalian sperm, where mitochondria wrap around the ciliary cytoskeleton. We find that mitochondria are tethered to their neighbors through inter-mitochondrial linkers and are anchored to the cytoskeleton through ordered arrays on the outer mitochondrial membrane. We use subtomogram averaging to resolve in-cell structures of these arrays from three mammalian species, revealing they are conserved across species despite variations in mitochondrial dimensions and cristae organization. We find that the arrays consist of boat-shaped particles anchored on a network of membrane pores whose arrangement and dimensions are consistent with voltage dependent anion channels. Proteomics and in-cell cross-linking mass spectrometry suggest that the conserved arrays are composed of glycerol kinase-like proteins. Ordered supramolecular assemblies may serve to stabilize similar contact sites in other cell types where mitochondria need to be immobilized in specific subcellular environments, such as in muscles and neurons.
HostingRepository	PRIDE
AnnounceDate	2021-10-29
AnnouncementXML	Submission_2021-10-29_01:51:47.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riccardo Zenezini Chiozzi
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-22 06:42:36	ID requested
⏵ 1	2021-10-29 01:51:47	announced

Dataset with its publication pending

submitter keyword: Proteomics, crosslinking, boar sperm, mitochondria, cryo-electron tomography

Albert Heck
contact affiliation	Biomolecular Mass Spectrometry & Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, The Netherlands
contact email	a.j.r.heck@uu.nl
lab head
Riccardo Zenezini Chiozzi
contact affiliation	Utrecht university
contact email	r.zenezinichiozzi@uu.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025562
     1. Label: PRIDE project
     2. Name: In-cell structures of a conserved supramolecular array at the mitochondria-cytoskeleton interface in mammalian sperm