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PXD025445-1

PXD025445 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOncogene Regulated Release of Extracellular Vesicles
DescriptionOncogenes can alter metabolism by changing the balance between anabolic and catabolic processes. However, how oncogenes regulate tumor cell biomass remains poorly understood. Using isogenic MCF10A cells transformed with nine different oncogenes, we show that specific oncogenes reduce the biomass of cancer cells by promoting EV release. While MYC and AURKB elicited the highest number of EVs, each oncogene selectively altered the protein composition of released EVs. Likewise, oncogenes alter secreted miRNAs. MYC overexpressing cells require ceramide, while AURKB require ESCRT to release high levels of EVs. We identify an inverse relationship between MYC upregulation and activation of the RAS/MEK/ERK signaling pathway for regulating EV release in some tumor cells. Finally, lysosome genes and activity are downregulated in the context of MYC and AURKB, suggesting that cellular contents instead of being degraded, were released via EVs. Thus, oncogene mediated biomass regulation via differential EV release is a new metabolic phenotype.
HostingRepositoryMassIVE
AnnounceDate2021-04-16
AnnouncementXMLSubmission_2021-04-16_15:48:57.066.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrett Phinney
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-16 12:48:21ID requested
12021-04-16 15:48:57announced
Publication List
no publication
Keyword List
submitter keyword: oncogenes, extracellular vesicles (EVs), ceramide, ESCRT, MYC, HRAS, AURKB, biomass, lysosome
Contact List
Andrei Goga
contact affiliationDepartment of Cell & Tissue Biology, University of California, San Francisco
contact emailAndrei.Goga@ucsf.edu
lab head
Brett Phinney
contact affiliationUC Davis
contact emailbrettsp@ucdavis.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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