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PXD025400-1

PXD025400 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranslational activators and mitoribosomal isoforms cooperate to mediate mRNA-specific translation in Schizosaccharomyces pombe mitochondria
DescriptionMitochondrial mRNAs encode key subunits of the oxidative phosphorylation complexes that produce energy for the cell. In Saccharomyces cerevisiae, mitochondrial translation is under the control of translational activators, specific to each mRNA. In Schizosaccharomyces pombe, which more closely resembles the human system by its mitochondrial DNA structure and physiology, most translational activators appear to be either lacking, or recruited for post-translational functions. By combining bioinformatics, genetic and biochemical approaches we identified two interacting factors, Cbp7 and Cbp8, controlling Cytb production in S. pombe. We show that their absence affects cytb mRNA stability and decreases the accumulation of the Cytb protein. We further identified two classes of Cbp7/Cbp8 partners and showed that they modulated Cytb or Cox1 synthesis. First, two isoforms of bS1m small mitoribosomal subunits, that appear mutually exclusive and confer translational specificity. Second, a complex of four proteins dedicated to Cox1 synthesis, which includes an RNA helicase that interacts with the mitochondrial ribosome. Our results suggest that S. pombecontains, in addition to complexes of translational activators, a heterogeneous population of mitochondrial ribosomes that could specifically modulate translation depending on the mRNA translated, in order to optimally balance the production of different respiratory complex subunits.
HostingRepositoryPRIDE
AnnounceDate2021-10-11
AnnouncementXMLSubmission_2021-10-11_04:50:24.393.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD025400
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Cornu
SpeciesList scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-14 06:11:15ID requested
12021-10-11 04:50:24announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Immunoprecipitation, LC-MSMS
Contact List
David Cornu
contact affiliationInstitute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France
contact emaildavid.cornu@i2bc.paris-saclay.fr
lab head
David Cornu
contact affiliationSICaPS, Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Univ. Paris-Saclay 91198 Gif-sur-Yvette Cedex, France
contact emaildavid.cornu@i2bc.paris-saclay.fr
dataset submitter
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Dataset FTP location
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