<<< Full experiment listing

PXD025397-1

PXD025397 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCombination of a microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids
DescriptionBrain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix (BEM) to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with BEM significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augmentation, and electrophysiological function of human brain organoids were further improved in a reproducible manner by dynamic culture in microfluidic chamber devices. Our engineering concept of reconstituting brain-mimetic microenvironments facilitates the development of a reliable culture platform for brain organoids, enabling effective modeling and drug development for human brain diseases.
HostingRepositoryPRIDE
AnnounceDate2021-06-02
AnnouncementXMLSubmission_2021-06-02_00:26:53.362.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSung-Hyun Jo
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-14 06:10:57ID requested
12021-06-02 00:26:53announced
22021-08-26 04:38:36announced2021-08-26: Updated publication reference for PubMed record(s): 34354063.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: brain extracellular matrix, microfluidic device, organoid
Contact List
Seung-Woo Cho
contact affiliationDepartment of Biotechnology, Stem cell and biomaterial engineering Lab, Yonsei University, Republic of Korea.
contact emailseungwoocho@yonsei.ac.kr
lab head
Sung-Hyun Jo
contact affiliationSoongsil university
contact emailsh20511@soongsil.ac.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025397
PRIDE project URI
Repository Record List
[ + ]