PXD025378-1
PXD025378 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evidence for a Nuclear Role for Drosophila Dlg as a Regulator of the NURF Complex |
| Description | Scrib, Dlg, and Lgl are basolateral regulators of epithelial polarity and tumor suppressors whose molecular mechanisms of action remain unclear. We used proximity biotinylation to identify proteins localized near Dlg in the Drosophila wing imaginal disc epithelium. In addition to expected membrane- and cytoskeleton-associated protein classes, nuclear proteins were prevalent in the resulting mass spectrometry data set, including all four members of the NURF chromatin remodeling complex. Subcellular fractionation demonstrated a nuclear pool of Dlg and proximity ligation confirmed its position near the NURF complex. Genetic analysis showed that NURF activity is also required for the overgrowth of dlg tumors, and this growth suppression correlated with a reduction in Hippo pathway gene expression. Together, these data suggest a nuclear role for Dlg in regulating chromatin and transcription through a more direct mechanism than previously thought. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-10-25 |
| AnnouncementXML | Submission_2021-10-25_12:48:36.679.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brett Phinney |
| SpeciesList | scientific name: Drosophila melanogaster; common name: fruit fly; NCBI TaxID: 7227; |
| ModificationList | Carbamidomethyl; Oxidation; TMT6plex |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-04-13 17:06:38 | ID requested | |
| ⏵ 1 | 2021-10-25 12:48:37 | announced |
Publication List
| Sharp KA, Khoury MJ, Wirtz-Peitz F, Bilder D, Dlg as a regulator of the NURF complex. Mol Biol Cell, 32(21):ar23(2021) [pubmed] |
Keyword List
| submitter keyword: NURF Complex |
Contact List
| David Bilder | |
|---|---|
| contact affiliation | Department of Molecular and Cell Biology, University of California-Berkeley, Berkeley CA |
| contact email | bilder@berkeley.edu |
| lab head | |
| Brett Phinney | |
| contact affiliation | UC Davis |
| contact email | brettsp@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087186/ |
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