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PXD025294-1

PXD025294 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens
DescriptionThe rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. Here, we explore the potential of targeted mass spectrometry based proteomics for the detection of SARS-CoV-2 proteins in both research and clinical samples. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) mass spectrometry. For Nucleocapsid the limit of detection was found to be in the mid-attomole range (0.9 x 10-12 g). Next, this PRM assay is applied to the detection of viral proteins in in vitro mucus substitutes, as well as in various clinical specimens such as nasopharyngeal swabs and sputum. In this proof-of-concept study SARS-CoV-2 proteins could unambiguously be detected in various clinical samples, suggesting that the sensitivity of this technology may be sufficiently high to further explore its potential role in diagnostics.
HostingRepositoryPRIDE
AnnounceDate2021-10-26
AnnouncementXMLSubmission_2021-10-26_01:55:57.570.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeroen Demmers
SpeciesList scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-09 03:43:08ID requested
12021-10-26 01:55:57announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Covid-19
submitter keyword: SARS-CoV-2, COVID-19, Nucleocapsid protein (NP, NCAP), targeted mass spectrometry, parallel reaction monitoring, PRM, LC-MS, limit of detection, AQUA, clinical specimen
Contact List
Jeroen Demmers
contact affiliationProteomics Center | Erasmus University Medical Center | Rotterdam, the Netherlands
contact emailj.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliationProteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact emailj.demmers@erasmusmc.nl
dataset submitter
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Dataset FTP location
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