PXD025157-1

PXD025157 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	High resolution time-lapse proteomic analysis of sporulation
Description	Bacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome changeover during sporulation, a time-lapse proteomic analysis was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively investigate the changeover of sporulation regulatory proteins, coat proteins and other proteins involved in sporulation and spore biogenesis. Protein co-expression analysis revealed four co-expressed modules. Modules brown and green are upregulated during sporulation and are annotated as associated with sporulation. Module blue, which is negatively correlated with modules brown and green, comprised ribosomal and metabolic proteins, and module yellow is co-expressed with module blue and modules brown & green. Notably, several proteins not belonging to any of the known transcription regulons were identified as co-expressed with modules brown and green. We speculate that they may also play roles during sporulation. Finally, amounts of some coat proteins, for example morphogenetic coat proteins, decreased late in sporulation. While we speculate on their possible role in guiding or helping assembly of other coat proteins, after which they can be disposed of, such a hypothesis remains to be experimentally addressed.
HostingRepository	MassIVE
AnnounceDate	2021-10-09
AnnouncementXML	Submission_2021-10-09_02:15:50.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gertjan Kramer
SpeciesList	scientific name: Bacillus subtilis;
ModificationList	Oxidation; Carbamidomethyl
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-03 03:02:02	ID requested
⏵ 1	2021-10-09 02:15:52	announced

Publication List

Tu Z, Dekker HL, Roseboom W, Swarge BN, Setlow P, Brul S, Kramer G, Sporulation. Int J Mol Sci, 22(17):(2021) [pubmed]

Tu Z, Dekker HL, Roseboom W, Swarge BN, Setlow P, Brul S, Kramer G, Sporulation. Int J Mol Sci, 22(17):(2021) [pubmed]

Keyword List

submitter keyword: Bacillus subtilis, Sporulation, quantitative mass spectrometry, proteomics

submitter keyword: Bacillus subtilis, Sporulation, quantitative mass spectrometry, proteomics

Contact List

Gertjan Kramer
contact affiliation	University of Amsterdam
contact email	gertjan.kramer@uva.nl
lab head
Gertjan Kramer
contact affiliation	Universiteit van Amsterdam
contact email	gertjan.kramer@uva.nl
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087154/