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PXD025157 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHigh resolution time-lapse proteomic analysis of sporulation
DescriptionBacillus subtilis vegetative cells switch to sporulation upon nutrient limitation. To investigate the proteome changeover during sporulation, a time-lapse proteomic analysis was performed in a cell population that was induced to sporulate synchronously. Here, we are the first to comprehensively investigate the changeover of sporulation regulatory proteins, coat proteins and other proteins involved in sporulation and spore biogenesis. Protein co-expression analysis revealed four co-expressed modules. Modules brown and green are upregulated during sporulation and are annotated as associated with sporulation. Module blue, which is negatively correlated with modules brown and green, comprised ribosomal and metabolic proteins, and module yellow is co-expressed with module blue and modules brown & green. Notably, several proteins not belonging to any of the known transcription regulons were identified as co-expressed with modules brown and green. We speculate that they may also play roles during sporulation. Finally, amounts of some coat proteins, for example morphogenetic coat proteins, decreased late in sporulation. While we speculate on their possible role in guiding or helping assembly of other coat proteins, after which they can be disposed of, such a hypothesis remains to be experimentally addressed.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGertjan Kramer
SpeciesList scientific name: Bacillus subtilis;
ModificationListOxidation; Carbamidomethyl
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-03 03:02:02ID requested
12021-10-09 02:15:52announced
Publication List
Tu Z, Dekker HL, Roseboom W, Swarge BN, Setlow P, Brul S, Kramer G, Sporulation. Int J Mol Sci, 22(17):(2021) [pubmed]
Keyword List
submitter keyword: Bacillus subtilis, Sporulation, quantitative mass spectrometry, proteomics
Contact List
Gertjan Kramer
contact affiliationUniversity of Amsterdam
contact emailgertjan.kramer@uva.nl
lab head
Gertjan Kramer
contact affiliationUniversiteit van Amsterdam
contact emailgertjan.kramer@uva.nl
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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