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PXD024925-1

PXD024925 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDiscovery of metal-binding proteins by thermal proteome profiling.
DescriptionMetal-binding proteins (MBPs) play diverse and critical functions in all living systems and many human diseases are closely related to dysfunctional MBPs. Here we developed a chemoproteomic method named METAL-TPP for global discovery of MBPs in proteomes, which operates by extracting metals from MBPs with chelators and logging the resulting structural perturbation of MBPs with thermal proteome profiling. We applied METAL-TPP to the human proteomes and identified 2856 proteins with significant thermal shifts, including not only many known MBPs but also 75 proteins without previous annotation of metal-binding activity.
HostingRepositoryiProX
AnnounceDate2021-03-22
AnnouncementXMLSubmission_2021-03-22_05:15:11.283.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXin Zeng
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-22 05:14:48ID requested
12021-03-22 05:15:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: metal-binding proteins, thermal proteome profiling
Contact List
Chu Wang
contact affiliationPeking University
contact emailchuwang@pku.edu.cn
lab head
Xin Zeng
contact affiliationPeking University
contact emailxinzeng@pku.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI