PXD024912 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | T. gondii TIR1 strain temperature range thermal profiling experiment |
Description | Toxoplasma gondii is a single-celled eukaryotic parasite that chronically infects a quarter of the global population. In recent years, phenotypic screens have identified compounds that block parasite replication. Unraveling the complexity of pathogenesis and molecular mechanisms and pathways perturbed by such compounds requires target deconvolution. Thermal proteome profiling (TPP), also known as the cellular thermal shift assay (CETSA), recently emerged as a method to identify small molecule–target interactions in living cells and cell extracts in a variety of organisms, including unicellular eukaryotic pathogens. Ligand binding induces a thermal stability shift—stabilizing or destabilizing proteins that change conformationally in response to the ligand—that can be measured by mass spectrometry (MS). Cells are incubated with different concentrations of ligand and heated, causing thermal denaturation of proteins. The soluble protein is extracted and quantified with multiplexed, quantitative MS, giving rise to thousands of thermal denaturation profiles. Proteins engaging the ligand can be identified by their concentration-dependent thermal shift. The protocol provided here can be used to identify compound-target interactions and assess the impact of environmental or genetic perturbation on the thermal stability of the proteom in T. gondii and other eukaryotic pathogens. Here, we generate a reference dataset of the thermal profiles of extracellular T. gondii tachyzoites. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:11:26.795.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD024912 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alice Herneisen |
SpeciesList | scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; phosphorylated residue; 6x(13)C labeled residue; monohydroxylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-22 04:36:43 | ID requested | |
1 | 2023-03-10 19:51:03 | announced | |
⏵ 2 | 2023-11-14 07:11:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: parasite,TPP, CETSA, Toxoplasma |
Contact List
Sebastian Lourido |
contact affiliation | Associate Professor, Whitehead Institute for Biomedical Research and Massachusettes Institute of Technology |
contact email | lourido@wi.mit.edu |
lab head | |
Alice Herneisen |
contact affiliation | Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology |
contact email | alice@wi.mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024912
- Label: PRIDE project
- Name: T. gondii TIR1 strain temperature range thermal profiling experiment