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PXD024830-1

PXD024830 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteogenomic Landscape of Uterine Leiomyomas from Hereditary Leiomyomatosis and Renal Cell Cancer Patients
DescriptionPathogenic mutations in fumarate hydratase (FH) drive hereditary leiomyomatosis and renal cell cancer (HLRCC) and increase the risk of developing uterine leiomyomas (ULMs). An integrated proteogenomic analysis of ULMs from HLRCC (n=16; FH-mutation confirmed) and non-syndromic (NS) patients (n=12) identified a significantly higher protein:transcript correlation in HLRCC (R=0.35) vs. NS ULMs (R=0.242, MWU p=0.0015). Co-altered proteins and transcripts (228) included antioxidant response element (ARE) target genes, such as thioredoxin reductase 1 (TXNRD1), and correlated with activation of NRF2-mediated oxidative stress response signaling in HLRCC ULMs. We confirm 185 transcripts previously described as altered between HLRCC and NS ULMs, 51 co-altered at the protein level and several elevated in HLRCC ULMs are involved in regulating cellular metabolism and glycolysis signaling. Furthermore, 367 S-(2-succino)cysteine peptides were identified in HLRCC ULMs, of which sixty were significantly elevated in HLRCC vs. NS ULMs (LogFC=1.86, MWU p<0.0001). These results confirm and define novel proteogenomic alterations in uterine leiomyoma tissues collected from HLRCC patients and underscore conserved molecular alterations correlating with inactivation of the FH tumor suppressor gene.
HostingRepositoryPRIDE
AnnounceDate2021-12-15
AnnouncementXMLSubmission_2021-12-15_03:29:23.368.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrian Hood
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-18 03:54:59ID requested
12021-12-15 03:29:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Hereditary Leiomyomatosis and Renal Cell Cancer, HLRCC, Uterine Leiomyoma, Fibroid, Proteogenomics, TMT-10, Quantitative Proteomics
Contact List
Thomas P Conrads, PhD
contact affiliationDirector of Women’s Health Research Women’s Service Line Inova Health System Chief Scientific Officer Gynecologic Cancer Center of Excellence John P. Murtha Cancer Center Research Program Uniformed Services University of the Health Sciences
contact emailconrads@whirc.org
lab head
Brian Hood
contact affiliationWomen's Health Integrated Research Center
contact emailhoodb@whirc.org
dataset submitter
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