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PXD024816-1

PXD024816 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMutation of Arabidopsis SME1 improves oxidative stress resilience
DescriptionAlternative splicing is an important posttranscriptional gene regulatory process, acting in diverse adaptive and basal plant processes. Splicing of precursor-messenger RNA (pre-mRNA) is catalyzed by a dynamic ribonucleoprotein complex, termed the spliceosome. In a suppressor screen, we identified a nonsense mutation in the Sm protein SME1 to alleviate photorespiratory H2O2-dependent cell death in catalase-deficient plants. Furthermore, sme1-2 mutants showed increased tolerance to the superoxide-generating herbicide methyl viologen. mRNA-seq and proteomic analyses indicated a constitutive stress response and pre-mRNA splicing alterations in sme1-2 mutants under control conditions. With a pulldown experiment, using SME1 as a bait, we provide experimental evidence for proteins associated with Arabidopsis thaliana spliceosome complexes, by identifying almost 50 proteins homologous to mammalian spliceosome-associated proteins and propose four novel proteins previously not-reported or hypothesized to be part of plant spliceosome complexes. Mutation of one of these interactors, the Sm core assembly protein ICLN also resulted in a decreased sensitivity to methyl viologen. Taken together, we show that SME1 mutation and impaired snRNP assembly result in enhanced resilience to oxidative stress.
HostingRepositoryPRIDE
AnnounceDate2023-03-03
AnnouncementXMLSubmission_2023-03-03_00:49:58.228.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPatrickWillems
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-18 01:02:37ID requested
12023-03-03 00:49:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis thaliana, spliceosome, CATALASE2, oxidative stress, Sm protein
Contact List
Frank VanBreusegem
contact affiliationDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
contact emailfrbre@psb.ugent.be
lab head
PatrickWillems
contact affiliationPSB-VIB
contact emailpawil@psb.ugent.be
dataset submitter
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Dataset FTP location
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