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PXD024808-1

PXD024808 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProfile of the SARS-CoV-2 RNA interactome
DescriptionSARS-CoV-2 is an RNA virus whose success as a pathogen relies on its ability to repurpose host RNA-binding proteins (RBPs) to form its own RNA interactome. Here, we developed and applied a robust ribonucleoprotein capture protocol to uncover the SARS-CoV-2 RNA interactome. We report 109 host factors that directly bind to SARS-CoV-2 RNAs including general antiviral factors such as ZC3HAV1, TRIM25, and PARP12. Applying RNP capture on another coronavirus HCoV-OC43 revealed evolutionarily conserved interactions between viral RNAs and host proteins. Network and transcriptome analyses delineated antiviral RBPs stimulated by JAK-STAT signaling and proviral RBPs responsible for hijacking multiple steps of the mRNA life cycle. By knockdown experiments, we further found that these viral-RNA-interacting RBPs act against or in favor of SARS-CoV-2. Overall, this study provides a comprehensive list of RBPs regulating coronaviral replication and opens new avenues for therapeutic interventions.
HostingRepositoryPRIDE
AnnounceDate2021-04-28
AnnouncementXMLSubmission_2021-04-27_22:03:44.125.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYeon Choi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-17 11:19:58ID requested
12021-04-27 22:03:44announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SARS-CoV-2, HCoV-OC43, Vero, HCT-8, RNA interactome, RBP, RNP
Contact List
V. Narry Kim
contact affiliationCenter for RNA Research, Institute for Basic Science, Seoul, Republic of Korea. School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
contact emailnarrykim@snu.ac.kr
lab head
Yeon Choi
contact affiliationSeoul National University
contact emailkitechoi9599@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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