PXD024808

PXD024808 is an original dataset announced via ProteomeXchange.

Title	Profile of the SARS-CoV-2 RNA interactome
Description	SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its ability to repurpose host RNA-binding proteins (RBPs) to form its own RNA interactome. Here, we developed and applied a robust ribonucleoprotein capture protocol to uncover the SARS-CoV-2 RNA interactome. We report 109 host factors that directly bind to SARS-CoV-2 RNAs including general antiviral factors such as ZC3HAV1, TRIM25, and PARP12. Applying RNP capture on another coronavirus HCoV-OC43 revealed evolutionarily conserved interactions between viral RNAs and host proteins. Network and transcriptome analyses delineated antiviral RBPs stimulated by JAK-STAT signaling and proviral RBPs responsible for hijacking multiple steps of the mRNA life cycle. By knockdown experiments, we further found that these viral-RNA-interacting RBPs act against or in favor of SARS-CoV-2. Overall, this study provides a comprehensive list of RBPs regulating coronaviral replication and opens new avenues for therapeutic interventions.
HostingRepository	PRIDE
AnnounceDate	2021-04-28
AnnouncementXML	Submission_2021-04-27_22:03:44.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yeon Choi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-17 11:19:58	ID requested
⏵ 1	2021-04-27 22:03:44	announced

Dataset with its publication pending

submitter keyword: SARS-CoV-2, HCoV-OC43, Vero, HCT-8, RNA interactome, RBP, RNP

V. Narry Kim
contact affiliation	Center for RNA Research, Institute for Basic Science, Seoul, Republic of Korea. School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
contact email	narrykim@snu.ac.kr
lab head
Yeon Choi
contact affiliation	Seoul National University
contact email	kitechoi9599@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD024808

PRIDE project URI

• PRIDE
  1. PXD024808
     1. Label: PRIDE project
     2. Name: Profile of the SARS-CoV-2 RNA interactome