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PXD024767-2

PXD024767 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title1. Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells
Description2. Enveloped viruses hijack not only host translation processes, but also its glycosylation machinery and to a variable extent cover viral surface proteins with tolerogenic host-like structures. SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported. The biosynthetic nature of O-glycosylation is highly variable and is affected by initiating enzyme repertoires in different tissues and cell types, and therefore warrants a more thorough investigation. Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S – insect cell or human cell-produced ectodomains, or insect cell-derived receptor binding domain (RBD). In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD. Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites. However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy. This suggests possible complimentary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2
HostingRepositoryPRIDE
AnnounceDate2021-04-07
AnnouncementXMLSubmission_2021-04-07_01:34:38.665.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSergey Vakhrushev
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Q Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-16 04:58:00ID requested
12021-04-07 01:11:41announced
22021-04-07 01:34:39announced2021-04-07: Updated project metadata.
Publication List
Bagdonaite I, Thompson AJ, Wang X, Søgaard M, Fougeroux C, Frank M, Diedrich JK, Yates JR, Salanti A, Vakhrushev SY, Paulson JC, Wandall HH, Site-Specific O-Glycosylation Analysis of SARS-CoV-2 Spike Protein Produced in Insect and Human Cells. Viruses, 13(4):(2021) [pubmed]
10.3390/V13040551;
Keyword List
ProteomeXchange project tag: Covid-19
submitter keyword: SARS-CoV-2, O-glycosylation, LC-MS, N-glycosylation, ETD
Contact List
Sergey Vakhrushev
contact affiliationUniversity of Copenhagen, Department of Cellular and Molecular Medicine, Copenhagen Center for Glycomics
contact emailseva@sund.ku.dk
lab head
Sergey Vakhrushev
contact affiliationDepartment of Cellular and Molecular Medicine
contact emailseva@sund.ku.dk
dataset submitter
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