PXD024677-1

PXD024677 is an original dataset announced via ProteomeXchange.

Title	Acute temperature-induced liver proteome changes in two populations of threespine stickleback (Gasterosteus aculeatus) revealed by data-independent acquisition mass spectrometry
Description	A data-independent acquisition assay library was used to examine proteome wide changes in liver of two populations of threespine stickleback (Gasterosteus aculeatus) after acute thermal stress. Experimental fish were held at 28°C for two hours and allowed to recover back at the acclimation temperature of 15°C for either six or 24 hours, while controls remained at 15°C. The Big Lagoon population (BL) had a large response at six hours after acute heat stress but had mostly regained protein homeostasis by 24 hours after the heat stress. The Klamath population showed very little response to acute thermal stress at either time point, highlighting major differences between the two populations in their response to acute heat stress. Functional enrichment analyses using STRING aided in identifying larger networks and domains that were significantly enriched in the different groups beyond the individual significantly different proteins, and KEGG pathway analysis was conducted on the large number of significantly different proteins for the BL28-6hr vs BL15-6hr comparison. DnaJ/Hsp40 B1b was the one protein that was significantly higher in abundance for both populations at both time points, suggesting a key role for this protein in regulating and orchestrating the response to acute temperature stress for this species. At six hours after acute heat stress, the main themes from the significant protein changes in the Big Lagoon population were the regulation of multiple levels of gene expression, reduction of reactive oxygen species (ROS) production, and structural changes.
HostingRepository	PanoramaPublic
AnnounceDate	2021-06-21
AnnouncementXML	Submission_2021-06-21_20:22:17.513.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bryn Levitan
SpeciesList	scientific name: Gasterosteus aculeatus; NCBI TaxID: 69293;
ModificationList	Acetyl; Oxidation; Carbamidomethyl
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 04:40:10	ID requested
⏵ 1	2021-06-21 20:22:17	announced

Dataset with its publication pending

submitter keyword: proteomics, acute temperature stress, liver, threespine stickleback

Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
lab head
Bryn Levitan
contact affiliation	UC Davis
contact email	bblevitan@ucdavis.edu
dataset submitter

Panorama Public dataset URI