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PXD024677 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAcute temperature-induced liver proteome changes in two populations of threespine stickleback (Gasterosteus aculeatus) revealed by data-independent acquisition mass spectrometry
DescriptionA data-independent acquisition assay library was used to examine proteome wide changes in liver of two populations of threespine stickleback (Gasterosteus aculeatus) after acute thermal stress. Experimental fish were held at 28°C for two hours and allowed to recover back at the acclimation temperature of 15°C for either six or 24 hours, while controls remained at 15°C. The Big Lagoon population (BL) had a large response at six hours after acute heat stress but had mostly regained protein homeostasis by 24 hours after the heat stress. The Klamath population showed very little response to acute thermal stress at either time point, highlighting major differences between the two populations in their response to acute heat stress. Functional enrichment analyses using STRING aided in identifying larger networks and domains that were significantly enriched in the different groups beyond the individual significantly different proteins, and KEGG pathway analysis was conducted on the large number of significantly different proteins for the BL28-6hr vs BL15-6hr comparison. DnaJ/Hsp40 B1b was the one protein that was significantly higher in abundance for both populations at both time points, suggesting a key role for this protein in regulating and orchestrating the response to acute temperature stress for this species. At six hours after acute heat stress, the main themes from the significant protein changes in the Big Lagoon population were the regulation of multiple levels of gene expression, reduction of reactive oxygen species (ROS) production, and structural changes.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBryn Levitan
SpeciesList scientific name: Gasterosteus aculeatus; NCBI TaxID: 69293;
ModificationListAcetyl; Oxidation; Carbamidomethyl
Instrumentimpact II
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-11 04:40:10ID requested
12021-06-21 20:22:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, acute temperature stress, liver, threespine stickleback
Contact List
Dietmar Kültz
contact affiliationUC Davis
contact emaildkueltz@ucdavis.edu
lab head
Bryn Levitan
contact affiliationUC Davis
contact emailbblevitan@ucdavis.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI