PXD024535

PXD024535 is an original dataset announced via ProteomeXchange.

Title	Liver proteomics investigations into hypothyroidism treated with Atractylodis Macrocephalae Rhizome
Description	Hypothyroidism model rats were established with PTU (10 mg•kg-1•d-1) and treated with Atractylodis Macrocephalae Rhizome and its fractions (volatile oil fraction, lactones fraction, atractyloside fraction, oligosaccharide fraction, and crude polysaccharide fraction) for 20 d. The experiment (having been) finished, liver samples were gained. Protein concentrations measured by the BCA kit and the tryptic peptides obtained from the different groups were labeled with iTRAQ reagents. The peptides were eluted into 10 fractions by High-pH Reverse-phase Liquid Chromatography Fractionation and analyzed by LC-ESI-MS/MS Analysis based on Triple TOF 5600. Results indicated that these DEPs contained Pygl, Pklr, Pc, Ehhadh, Acox1, Fasn, Acly, Acsl1, Acsl5, Pgm1, Suclg1, Gpt, Idh1, Fh, and Adh1, which could represent target proteins of Atractylodis Macrocephalae Rhizome and its fractions for hypothyroidism. Atractylodis Macrocephalae Rhizome and its fractions played roles in regulating the substance and energy metabolism for the treatment of hypothyroidism.
HostingRepository	iProX
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2023-02-19_23:40:40.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Chen
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-04 19:34:02	ID requested
1	2021-03-04 19:34:31	announced
⏵ 2	2023-02-19 23:40:41	announced	2023-02-20: Update publication information.

Chen J, Dou P, Xiao H, Dou D, Han X, Kuang H, Application of Proteomics and Metabonomics to Reveal the Molecular Basis of Atractylodis Macrocephalae Rhizome for Ameliorating Hypothyroidism Instead of Hyperthyroidism. Front Pharmacol, 12():664319(2021) [pubmed]

submitter keyword: Hypothyroidism, Atractylodis Macrocephalae Rhizome

Deqiang Dou
contact affiliation	Liaoning University of Traditional Chinese Medicine
contact email	deqiangdou@126.com
lab head
Jing Chen
contact affiliation	Liaoning University of Traditional Chinese Medicine
contact email	jingzai2012@163.com
dataset submitter

iProX dataset URI