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PXD024398-1

PXD024398 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic landscape of SARS-CoV-2- and MERS-CoV-infected primary human renal epithelial cells
DescriptionAcute kidney injury is strongly associated with mortality in COVID-19 patients. However, host cell changes underlying infection of kidney cells with SARS-CoV-2 remain unknown and prevent understanding of the mechanisms leading to renal pathology. Here, we carried out extensive, dual-level proteomic analyses of primary human kidney cells infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type specific changes. We established cellular infection models of proximal and distal tubular epithelial cells derived from human donors. Infected cells were analyzed by quantitative translatome and whole-cell proteomics over time. Our findings reveal shared pathways modified upon infection with both viruses, as well as SARS-CoV-2 specific host cell modulation driving key changes in innate immune activation, cellular protein quality control and mitochondrial biology. In addition, we identified extensive modulation in pathways associated with kidney failure that were instinctively regulated in a virus- and cell type-specific manner. Our findings provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary kidney cells revealing key pathways and highlighting potential treatment options.
HostingRepositoryPRIDE
AnnounceDate2022-01-30
AnnouncementXMLSubmission_2022-01-30_14:00:04.003.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAneesha Kohli
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; 6x(13)C labeled residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-26 04:50:44ID requested
12022-01-30 14:00:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: human, mePROD, TMD, TMT, coronavirus, SARS-CoV-2, MERS-CoV
Contact List
Christian Münch
contact affiliationInstitute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany
contact emailch.muench@em.uni-frankfurt.de
lab head
Aneesha Kohli
contact affiliationInstitute of Biochemistry II, Goethe University, Frankfurt am Main
contact emailaneeshak19@gmail.com
dataset submitter
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Dataset FTP location
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