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PXD024387-1

PXD024387 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry hits for GFP::BBLN-1/bioGFP::BBLN1 pull down
DescriptionEpithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer important characteristics to tubular organs and need to be carefully controlled. Here, we identify the small coiled-coil protein BBLN-1 as a regulator of lumen morphology in the C. elegans intestine. Loss of BBLN-1 causes the formation of bubble-shaped invaginations of the apical membrane into the cytoplasm of intestinal cells, and abnormal aggregation of the subapical intermediate filament (IF) network. BBLN-1 interacts with IF proteins and localizes to the IF network in an IF-dependent manner. The appearance of invaginations is a result of the abnormal IF aggregation, indicating a direct role for the IF network in maintaining lumen homeostasis. Finally, we identify bublin (BBLN) as the mammalian ortholog of BBLN-1. When expressed in the C. elegans intestine, bublin recapitulates the localization pattern of BBLN-1 and can compensate for the loss of BBLN-1. In mouse intestinal organoids, bublin localizes subapically, together with the IF protein keratin 8. Our results therefore may have implications for understanding the role of IFs in regulating epithelial tube morphology in mammals.
HostingRepositoryPRIDE
AnnounceDate2021-04-07
AnnouncementXMLSubmission_2021-04-07_01:32:20.863.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSanne Remmelzwaal
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-25 05:56:56ID requested
12021-04-07 01:32:21announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: C. elegans, BBLN-1, HEK293T, bublin, affinity purification, mass spectrometry
Contact List
Mike Boxem
contact affiliationDevelopmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
contact emailm.boxem@uu.nl
lab head
Sanne Remmelzwaal
contact affiliationUtrecht University
contact emails.m.remmelzwaal@uu.nl
dataset submitter
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