PXD024387 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry hits for GFP::BBLN-1/bioGFP::BBLN1 pull down |
Description | Epithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer important characteristics to tubular organs and need to be carefully controlled. Here, we identify the small coiled-coil protein BBLN-1 as a regulator of lumen morphology in the C. elegans intestine. Loss of BBLN-1 causes the formation of bubble-shaped invaginations of the apical membrane into the cytoplasm of intestinal cells, and abnormal aggregation of the subapical intermediate filament (IF) network. BBLN-1 interacts with IF proteins and localizes to the IF network in an IF-dependent manner. The appearance of invaginations is a result of the abnormal IF aggregation, indicating a direct role for the IF network in maintaining lumen homeostasis. Finally, we identify bublin (BBLN) as the mammalian ortholog of BBLN-1. When expressed in the C. elegans intestine, bublin recapitulates the localization pattern of BBLN-1 and can compensate for the loss of BBLN-1. In mouse intestinal organoids, bublin localizes subapically, together with the IF protein keratin 8. Our results therefore may have implications for understanding the role of IFs in regulating epithelial tube morphology in mammals. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-07 |
AnnouncementXML | Submission_2021-04-07_01:32:20.863.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sanne Remmelzwaal |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-25 05:56:56 | ID requested | |
⏵ 1 | 2021-04-07 01:32:21 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: C. elegans, BBLN-1, HEK293T, bublin, affinity purification, mass spectrometry |
Contact List
Mike Boxem |
contact affiliation | Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands |
contact email | m.boxem@uu.nl |
lab head | |
Sanne Remmelzwaal |
contact affiliation | Utrecht University |
contact email | s.m.remmelzwaal@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD024387
- Label: PRIDE project
- Name: Mass spectrometry hits for GFP::BBLN-1/bioGFP::BBLN1 pull down