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PXD024381-1

PXD024381 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCorynebacterium diphtheriae proteome adaptation to cell culture medium and serum
Descriptionost-pathogen interactions are often studied in vitro using primary or immortal cell lines. This set-up avoids ethical problems of animal testing and has the additional advantage of lower costs. However, the influence of cell culture media on bacterial growth and metabolism is not considered or investigated in most cases. To address this question growth and proteome adaptation of Corynebacterium diphtheriae strain ISS3319 were investigated in this study. Bacteria were cultured in standard growth medium, cell culture medium and fetal calf serum. Mass spectrometric analyses and label-free protein quantification hint to an increased bacterial pathogenicity when grown in cell culture medium as well as an influence of the growth medium on the cell envelope.
HostingRepositoryPRIDE
AnnounceDate2021-03-12
AnnouncementXMLSubmission_2021-03-12_00:00:52.858.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJens Möller
SpeciesList scientific name: Corynebacterium diphtheriae bv. mitis str. ISS 3319; NCBI TaxID: 1450520;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-25 02:57:48ID requested
12021-03-12 00:00:53announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: diphtheria, host-pathogen interaction, label-free, metabolic pathway, proteomics
Contact List
Andreas Burkovski
contact affiliationDepartment Biologie Professur für Mikrobiologie (Prof. Dr. Burkovski)
contact emailandreas.burkovski@fau.de
lab head
Jens Möller
contact affiliationFAU Erlangen
contact emailJens.moeller@fau.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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