PXD024366-1

PXD024366 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of the ciliary Hedgehog response reveals coordinated exit of GPR161 and PKA
Description	The primary cilium is a signaling compartment that interprets Hedgehog signals through changes of its protein, lipid and second messenger compositions. Here, we combine proximity labeling of cilia with quantitative mass spectrometry to unbiasedly profile the time-dependent alterations of the ciliary proteome in response to Hedgehog. This approach correctly identifies the three factors known to undergo Hedgehog-regulated ciliary redistribution and reveals two such additional proteins. First, we find that a regulatory subunit of the cAMP-dependent protein kinase (PKA) rapidly exits cilia together with the G protein-coupled receptor GPR161 in response to Hedgehog; and we propose that the GPR161/PKA module senses and amplifies cAMP signals to modulate ciliary PKA activity. Second, we identify the phosphatase Paladin as a cell type-specific regulator of Hedgehog signaling that enters primary cilia upon pathway activation. The broad applicability of quantitative ciliary proteome profiling promises a rapid characterization of ciliopathies and their underlying signaling malfunctions.
HostingRepository	PRIDE
AnnounceDate	2021-04-07
AnnouncementXML	Submission_2021-04-06_22:49:25.485.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marian Kalocsay
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 10:32:42	ID requested
⏵ 1	2021-04-06 22:49:25	announced

Dataset with its publication pending

submitter keyword: Primary Cilium, protein trafficking, proximity labeling, proteomics, hedgehog signaling

David Mick
contact affiliation	Center of Human and Molecular Biology (ZHMB), Saarland University School of Medicine, Homburg, Germany
contact email	david.mick@uks.eu
lab head
Marian Kalocsay
contact affiliation	Harvard Medical School
contact email	marian_kalocsay@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024366
     1. Label: PRIDE project
     2. Name: Proteomic profiling of the ciliary Hedgehog response reveals coordinated exit of GPR161 and PKA