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PXD024366

PXD024366 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic profiling of the ciliary Hedgehog response reveals coordinated exit of GPR161 and PKA
DescriptionThe primary cilium is a signaling compartment that interprets Hedgehog signals through changes of its protein, lipid and second messenger compositions. Here, we combine proximity labeling of cilia with quantitative mass spectrometry to unbiasedly profile the time-dependent alterations of the ciliary proteome in response to Hedgehog. This approach correctly identifies the three factors known to undergo Hedgehog-regulated ciliary redistribution and reveals two such additional proteins. First, we find that a regulatory subunit of the cAMP-dependent protein kinase (PKA) rapidly exits cilia together with the G protein-coupled receptor GPR161 in response to Hedgehog; and we propose that the GPR161/PKA module senses and amplifies cAMP signals to modulate ciliary PKA activity. Second, we identify the phosphatase Paladin as a cell type-specific regulator of Hedgehog signaling that enters primary cilia upon pathway activation. The broad applicability of quantitative ciliary proteome profiling promises a rapid characterization of ciliopathies and their underlying signaling malfunctions.
HostingRepositoryPRIDE
AnnounceDate2021-04-07
AnnouncementXMLSubmission_2021-04-06_22:49:25.485.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarian Kalocsay
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-24 10:32:42ID requested
12021-04-06 22:49:25announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Primary Cilium, protein trafficking, proximity labeling, proteomics, hedgehog signaling
Contact List
David Mick
contact affiliationCenter of Human and Molecular Biology (ZHMB), Saarland University School of Medicine, Homburg, Germany
contact emaildavid.mick@uks.eu
lab head
Marian Kalocsay
contact affiliationHarvard Medical School
contact emailmarian_kalocsay@hms.harvard.edu
dataset submitter
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