PXD024242 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of the apical secretome of Calu-3 and NHBE cells in 3D models of the human bronchial epithelium |
Description | The mucus secreted by airway epithelial cells plays a key role in the protection against and clearance of particles and pathogens. In this project, a 3D model of the bronchial epithelium was established using Calu-3 cells grown on porous inserts at the air-liquid interface. The secreted proteins were characterized in long-term cultures and compared to the apical secretome of primary normal human bronchial epithelial (NHBE) cells. The apical secretome was collected and characterized in the Calu-3 model at day 4, day 11, and day 18 after air-liquid interface and in the NHBE model at day 4, day 12, and day 18 after air-liquid interface. "This project received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement no 760928 (BIORIMA)." |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:52:11.308.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniel Sanchez-Guzman |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-18 10:04:49 | ID requested | |
1 | 2021-03-26 09:47:39 | announced | |
⏵ 2 | 2023-11-14 08:52:11 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, LC-MSMS, Calu-3, NHBE, apical secretome, epithelial cells |
Contact List
Armelle Baeza Squiban |
contact affiliation | Université de Paris, BFA unit UMR 8251 CNRS |
contact email | armelle.baeza@u-paris.fr |
lab head | |
Daniel Sanchez-Guzman |
contact affiliation | Equipe 6 BFA, Université de Paris |
contact email | daniel.sanchez-guzman@u-paris.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024242 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024242
- Label: PRIDE project
- Name: Analysis of the apical secretome of Calu-3 and NHBE cells in 3D models of the human bronchial epithelium