PXD024242

PXD024242 is an original dataset announced via ProteomeXchange.

Title	Analysis of the apical secretome of Calu-3 and NHBE cells in 3D models of the human bronchial epithelium
Description	The mucus secreted by airway epithelial cells plays a key role in the protection against and clearance of particles and pathogens. In this project, a 3D model of the bronchial epithelium was established using Calu-3 cells grown on porous inserts at the air-liquid interface. The secreted proteins were characterized in long-term cultures and compared to the apical secretome of primary normal human bronchial epithelial (NHBE) cells. The apical secretome was collected and characterized in the Calu-3 model at day 4, day 11, and day 18 after air-liquid interface and in the NHBE model at day 4, day 12, and day 18 after air-liquid interface. "This project received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement no 760928 (BIORIMA)."
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:11.308.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Sanchez-Guzman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-18 10:04:49	ID requested
1	2021-03-26 09:47:39	announced
⏵ 2	2023-11-14 08:52:11	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, LC-MSMS, Calu-3, NHBE, apical secretome, epithelial cells

Armelle Baeza Squiban
contact affiliation	Université de Paris, BFA unit UMR 8251 CNRS
contact email	armelle.baeza@u-paris.fr
lab head
Daniel Sanchez-Guzman
contact affiliation	Equipe 6 BFA, Université de Paris
contact email	daniel.sanchez-guzman@u-paris.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024242

PRIDE project URI

• PRIDE
  1. PXD024242
     1. Label: PRIDE project
     2. Name: Analysis of the apical secretome of Calu-3 and NHBE cells in 3D models of the human bronchial epithelium