PXD024220 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The structure of a virus-encoded nucleosome |
Description | Eukaryotes and prokaryotes encode proteins with the histone-fold domain, but only eukaryotes are known to encode "core histones" from four distinct families that heterodimerize selectively and uniquely to form nucleosome particles. Some large DNA viruses, including those in Marseilleviridae family encode histones. The presence of histone proteins in viral particles suggests their role in compacting viral genomes but it is not known whether these histones can form higher-order structures like those found in eukaryotes. Here we show that fused histone pairs Hβ-Hα and Hδ-Hγ from Marseillevirus are structurally analogous to the eukaryotic histone pairs H2B-H2A and H3-H4. We further show that they form “forced” heterodimers and that a heterotetramer of four such heterodimers assembles DNA to form structures virtually identical to those of canonical eukaryotic nucleosomes. We characterize these viral structures using cryo-EM to reveal their unprecedented conservation with the nucleosome—one of the most important and conserved features of eukaryotic chromosomes. The structure and properties of these viral nucleosomes hold clues to understanding the origins of eukaryotic chromatin. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-28 |
AnnouncementXML | Submission_2021-05-27_22:28:25.329.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nikita Vasilyev |
SpeciesList | scientific name: Marseillevirus; NCBI TaxID: 1513458; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-17 10:22:39 | ID requested | |
1 | 2021-05-27 09:52:10 | announced | |
⏵ 2 | 2021-05-27 22:28:26 | announced | 2021-05-28: Updated project metadata. |
Publication List
10.1038/S41594-021-00585-7; |
Valencia-S, á, nchez MI, Abini-Agbomson S, Wang M, Lee R, Vasilyev N, Zhang J, De Ioannes P, La Scola B, Talbert P, Henikoff S, Nudler E, Erives A, Armache KJ, The structure of a virus-encoded nucleosome. Nat Struct Mol Biol, 28(5):413-417(2021) [pubmed] |
Keyword List
submitter keyword: Chromatin, Marseillevirus, Nucleosomes, Cryo-EM, Evolution, Histones |
Contact List
Evgeny Nudler |
contact affiliation | Department of Biochemistry and Molecular Pharmacology, NYU Grossman School of Medicine, New York, US |
contact email | evgeny.nudler@nyulangone.org |
lab head | |
Nikita Vasilyev |
contact affiliation | NYU School of Medicine |
contact email | nikita.vasilyev@nyulangone.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024220
- Label: PRIDE project
- Name: The structure of a virus-encoded nucleosome