PXD024220

PXD024220 is an original dataset announced via ProteomeXchange.

Title	The structure of a virus-encoded nucleosome
Description	Eukaryotes and prokaryotes encode proteins with the histone-fold domain, but only eukaryotes are known to encode "core histones" from four distinct families that heterodimerize selectively and uniquely to form nucleosome particles. Some large DNA viruses, including those in Marseilleviridae family encode histones. The presence of histone proteins in viral particles suggests their role in compacting viral genomes but it is not known whether these histones can form higher-order structures like those found in eukaryotes. Here we show that fused histone pairs Hβ-Hα and Hδ-Hγ from Marseillevirus are structurally analogous to the eukaryotic histone pairs H2B-H2A and H3-H4. We further show that they form “forced” heterodimers and that a heterotetramer of four such heterodimers assembles DNA to form structures virtually identical to those of canonical eukaryotic nucleosomes. We characterize these viral structures using cryo-EM to reveal their unprecedented conservation with the nucleosome—one of the most important and conserved features of eukaryotic chromosomes. The structure and properties of these viral nucleosomes hold clues to understanding the origins of eukaryotic chromatin.
HostingRepository	PRIDE
AnnounceDate	2021-05-28
AnnouncementXML	Submission_2021-05-27_22:28:25.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikita Vasilyev
SpeciesList	scientific name: Marseillevirus; NCBI TaxID: 1513458;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-17 10:22:39	ID requested
1	2021-05-27 09:52:10	announced
⏵ 2	2021-05-27 22:28:26	announced	2021-05-28: Updated project metadata.

10.1038/S41594-021-00585-7;

Valencia-S, á, nchez MI, Abini-Agbomson S, Wang M, Lee R, Vasilyev N, Zhang J, De Ioannes P, La Scola B, Talbert P, Henikoff S, Nudler E, Erives A, Armache KJ, The structure of a virus-encoded nucleosome. Nat Struct Mol Biol, 28(5):413-417(2021) [pubmed]

submitter keyword: Chromatin, Marseillevirus, Nucleosomes, Cryo-EM, Evolution, Histones

Evgeny Nudler
contact affiliation	Department of Biochemistry and Molecular Pharmacology, NYU Grossman School of Medicine, New York, US
contact email	evgeny.nudler@nyulangone.org
lab head
Nikita Vasilyev
contact affiliation	NYU School of Medicine
contact email	nikita.vasilyev@nyulangone.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024220

PRIDE project URI

• PRIDE
  1. PXD024220
     1. Label: PRIDE project
     2. Name: The structure of a virus-encoded nucleosome