PXD024172

PXD024172 is an original dataset announced via ProteomeXchange.

Title	CAF-1 promotes HIV-1 latency by leading the formation of phase-separated suppressive nuclear bodies
Description	HIV-1 latency is a major obstacle to achieve a functional cure for AIDS. To eradicate the viral reservoir, one of the strategies is to specifically reactivate HIV-1-infected cells and followed by elimination with potent immune surveillance. However, present latency-reversing agents (LRAs) are quite dissatisfactory because of their high toxicity and low efficiency. New targets need to be identified to develop more promising LRAs. Here, we found that histone chaperone chromatin assembly factor 1 (CAF-1) promotes HIV-1 latency by forming versatile suppressive nuclear bodies. CAF-1 plays a leading role for the formation of bodies recruiting multi-layer suppressive epigenetic modifiers and maintainers, and is of the characteristic of liquid-liquid phase separation (LLPS). Three distinct disordered regions of CHAF1A subunit coalesce CAF-1 body components by forming LLPS and play a key in maintaining HIV-1 latency. Therefore, disruptive induction of phase-separated CAF-1 body could be an important strategy to reactivate latent HIV-1.
HostingRepository	iProX
AnnounceDate	2021-02-09
AnnouncementXML	Submission_2021-04-22_17:22:53.306.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiancai Ma
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-15 00:58:05	ID requested
1	2021-02-15 00:58:34	announced
⏵ 2	2021-04-22 17:22:54	announced	2021-04-23: Update publication information.

Ma X, Chen T, Peng Z, Wang Z, Liu J, Yang T, Wu L, Liu G, Zhou M, Tong M, Guan Y, Zhang X, Lin Y, Tang X, Li L, Tang Z, Pan T, Zhang H, Histone chaperone CAF-1 promotes HIV-1 latency by leading the formation of phase-separated suppressive nuclear bodies. EMBO J, 40(10):e106632(2021) [pubmed]

submitter keyword: CAF-1

Hui Zhang
contact affiliation	Sun Yat-sen University
contact email	zhangh92@mail.sysu.edu.cn
lab head
Xiancai Ma
contact affiliation	Sun Yat-sen University
contact email	maxc@mail2.sysu.edu.cn
dataset submitter

iProX dataset URI