PXD024144 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Waves of sumoylation support transcription dynamics during adipocyte differentiation |
Description | Tight control of gene expression networks involved in adipose tissue formation and plasticity is required to adapt to energy needs and environmental cues. However, little is known about the mechanisms that orchestrate the dramatic transcriptional changes leading to adipocyte differentiation. We investigated the regulation of nascent transcription by SUMO during adipocyte differentiation using SLAMseq and ChIPseq. We discovered that SUMO has a dual function in differentiation; it supports the initial downregulation of pre-adipocyte-specific genes, while it promotes the establishment of the mature adipocyte transcriptional program. By characterizing SUMOylome dynamics in differentiating adipocytes by mass spectrometry, we found that SUMOylation of specific transcription factors like PPARG/RXR and chromatin modifiers promotes the transcription of adipogenic genes. Our data demonstrate that the sumoylation pathway helps coordinates the rewiring of transcriptional networks required for formation of functional adipocytes. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-18 |
AnnouncementXML | Submission_2022-02-18_07:53:22.145.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ivo Hendriks |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | sumoylated lysine |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-11 09:00:51 | ID requested | |
⏵ 1 | 2022-02-18 07:53:22 | announced | |
2 | 2023-05-14 14:21:23 | announced | 2023-05-14: Updated project metadata. |
Publication List
Zhao X, Hendriks IA, Le Gras S, Ye T, Ramos-Alonso L, Ngu, é, a P A, Lien GF, Ghasemi F, Klungland A, Jost B, Enserink JM, Nielsen ML, Chymkowitch P, Waves of sumoylation support transcription dynamics during adipocyte differentiation. Nucleic Acids Res, 50(3):1351-1369(2022) [pubmed] |
Keyword List
submitter keyword: SUMO, endogenous, native, adipose tissue, adipocyte, differentiation, transcription, chromatin |
Contact List
Michael Lund Nielsen |
contact affiliation | Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark |
contact email | michael.lund.nielsen@cpr.ku.dk |
lab head | |
Ivo Hendriks |
contact affiliation | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen |
contact email | ivo.a.hendriks@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024144
- Label: PRIDE project
- Name: Waves of sumoylation support transcription dynamics during adipocyte differentiation