PXD024144

PXD024144 is an original dataset announced via ProteomeXchange.

Title	Waves of sumoylation support transcription dynamics during adipocyte differentiation
Description	Tight control of gene expression networks involved in adipose tissue formation and plasticity is required to adapt to energy needs and environmental cues. However, little is known about the mechanisms that orchestrate the dramatic transcriptional changes leading to adipocyte differentiation. We investigated the regulation of nascent transcription by SUMO during adipocyte differentiation using SLAMseq and ChIPseq. We discovered that SUMO has a dual function in differentiation; it supports the initial downregulation of pre-adipocyte-specific genes, while it promotes the establishment of the mature adipocyte transcriptional program. By characterizing SUMOylome dynamics in differentiating adipocytes by mass spectrometry, we found that SUMOylation of specific transcription factors like PPARG/RXR and chromatin modifiers promotes the transcription of adipogenic genes. Our data demonstrate that the sumoylation pathway helps coordinates the rewiring of transcriptional networks required for formation of functional adipocytes.
HostingRepository	PRIDE
AnnounceDate	2023-05-14
AnnouncementXML	Submission_2023-05-14_14:21:23.058.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	IvoHendriks
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	sumoylated lysine
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-11 09:00:51	ID requested
1	2022-02-18 07:53:22	announced
⏵ 2	2023-05-14 14:21:23	announced	2023-05-14: Updated project metadata.

Zhao X, Hendriks IA, Le Gras S, Ye T, Ramos-Alonso L, Ngu, é, a P A, Lien GF, Ghasemi F, Klungland A, Jost B, Enserink JM, Nielsen ML, Chymkowitch P, Waves of sumoylation support transcription dynamics during adipocyte differentiation. Nucleic Acids Res, 50(3):1351-1369(2022) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: transcription,SUMO, adipocyte, endogenous, differentiation, adipose tissue, native, chromatin

Michael LundNielsen
contact affiliation	Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark
contact email	michael.lund.nielsen@cpr.ku.dk
lab head
IvoHendriks
contact affiliation	Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
contact email	ivo.a.hendriks@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD024144

PRIDE project URI

• PRIDE
  1. PXD024144
     1. Label: PRIDE project
     2. Name: Waves of sumoylation support transcription dynamics during adipocyte differentiation