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PXD024134-2

PXD024134 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOptimized Sample Preparation Workflow for Improved Identification of Ghost Proteins
DescriptionLarge scale proteomic strategies rely on database interrogation. Thus, only referenced proteins can be identified. Recently, Alternative Proteins (AltProts) translated from nonannotated Alternative Open reading frame (AltORFs) were discovered using customized databases. Because of their small size which confers them peptide-like physicochemical properties, they are more difficult to detect using standard proteomics strategies. In this study, we tested different preparation workflows for improving the identification of AltProts in NCH82 human glioma cell line. The highest number of identified AltProts was achieved with RIPA buffer or boiling water extraction followed by acetic acid precipitation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:52:10.922.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTristan Cardon
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-11 03:35:52ID requested
12021-02-15 08:20:13announced
22023-11-14 08:52:11announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Protein identification,Fractionation,Precipitation,Peptides and proteins,Extraction
Contact List
Michel Salzet
contact affiliationLaboratory PRISM-Inserm U1192 university of lille France
contact emailmichel.salzet@univ-lille.fr
lab head
Tristan Cardon
contact affiliationLaboratoire PRISM INSERM U1192
contact emailtristan.cardon@univ-lille.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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