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PXD024035-1

PXD024035 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOrphan nuclear receptor COUP-TFII enhances myofibroblasts glycolysis leading to kidney fibrosis
DescriptionRecent studies demonstrated that metabolic disturbance, such as augmented glycolysis, contributes to fibrosis. The molecular regulation of this metabolic perturbation in fibrosis, however, has been elusive. COUP-TFII (also known as NR2F2) is an important regulator of glucose and lipid metabolism. Its contribution to organ fibrosis is undefined. Here, we found increased COUP-TFII expression in myofibroblasts in human fibrotic kidneys, lungs, kidney organoids, and mouse kidneys after injury. Genetic ablation of COUP-TFII in mice resulted in attenuation of injury-induced kidney fibrosis. A non-biased proteomic study revealed the suppression of fatty acid oxidation and the enhancement of glycolysis pathways in COUP-TFII overexpressing fibroblasts. Overexpression of COUP-TFII in fibroblasts induced augmented glycolysis and production of alpha smooth muscle actin (αSMA) and collagen1. Knockout of COUP-TFII decreased glycolysis and collagen1 levels in fibroblasts. Chip-qPCR revealed the binding of COUP-TFII on the promoter of PGC1α. Overexpression of COUP-TFII reduced the cellular level of PGC1α. Targeting COUP-TFII serves as a novel treatment approach for mitigating fibrosis in chronic kidney disease and potentially fibrosis in other organs.
HostingRepositoryPRIDE
AnnounceDate2021-04-27
AnnouncementXMLSubmission_2021-04-26_22:13:23.549.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarian Kalocsay
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-07 23:59:29ID requested
12021-04-26 22:13:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Kidney, Fibrosis, COUP-TFII, myofibroblasts
Contact List
Li Li
contact affiliationRenal Division, Brigham and Women’s Hospital, Boston, Massachusetts, USA. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
contact emaillli29@bwh.harvard.edu
lab head
Marian Kalocsay
contact affiliationHarvard Medical School
contact emailmarian_kalocsay@hms.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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