PXD024033-1

PXD024033 is an original dataset announced via ProteomeXchange.

Title	Targets of kinase/phosphatase signalling pair StkP/PhpP in S. pneumoniae and their involvement in response to cell wall stress
Description	Streptococcus pneumoniae is an opportunistic human pathogen which encodes a single eukaryotic-type Ser/Thr protein kinase StkP and its functional counterpart protein phosphatase PhpP. These signalling enzymes play a crucial role in the coordination of cell division and growth in pneumococcus. In this study, we determined the proteomic and phosphoproteomic profiles of relevant mutants. A comparison with the wild type provided a representative data set of new phosphoacceptor sites and StkP-dependent substrates. StkP phosphorylates key proteins involved in cell division and cell wall biosynthesis in unencapsulated laboratory Rx1 strain as well as encapsulated virulent strain D39. Moreover, we show that StkP plays important role in eliciting an adaptive response induced by cell wall directed antibiotic. Phosphorylation of WalK sensor kinase and decline of the abundance of WalK and proteins of core WalR/K regulon implies cross-talk between StkP and WalR/K two-component system. Inspection of proteome profiles revealed gene clusters regulated by catabolite control mechanism suggesting a tight coupling of carbon flow and cell wall homeostasis. The disbalance of steady-state protein phosphorylation in the mutants as well as after antibiotic treatment is accompanied by accumulation of global Spx regulator indicating Spx-mediated envelope stress response. In summary, StkP translates the sensed signal about the cell-wall status to key cell division and regulatory proteins and in this way controls cell cycle and cell wall homeostasis.
HostingRepository	PRIDE
AnnounceDate	2022-05-12
AnnouncementXML	Submission_2022-05-12_06:17:16.893.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivo Fabrik
SpeciesList	scientific name: Streptococcus pneumoniae R6; NCBI TaxID: 171101; scientific name: Streptococcus pneumoniae D39; NCBI TaxID: 373153;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-07 23:56:32	ID requested
⏵ 1	2022-05-12 06:17:17	announced
2	2023-11-14 08:51:35	announced	2023-11-14: Updated project metadata.

Ulrych A, Fabrik I, Kup, č, í, k R, Vajrychov, á M, Doubravov, á L, Branny P, Cell Wall Stress Stimulates the Activity of the Protein Kinase StkP of Streptococcus pneumoniae, Leading to Multiple Phosphorylation. J Mol Biol, 433(24):167319(2021) [pubmed]

10.1016/J.JMB.2021.167319;

submitter keyword: Streptococcus pneumoniae, Ser/Thr protein kinase StkP, phosphatase PhpP, phosphoproteome, cell wall stress, ampicillin treatment, signalling

Pavel Branny
contact affiliation	Institute of Microbiology of the CAS, v. v. i., Videnska 1083, 142 20 Prague, Czech Republic
contact email	branny@biomed.cas.cz
lab head
Ivo Fabrik
contact affiliation	Biomedical Research Center, University Hospital Hradec Kralove
contact email	ivo.fabrik@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024033
     1. Label: PRIDE project
     2. Name: Targets of kinase/phosphatase signalling pair StkP/PhpP in S. pneumoniae and their involvement in response to cell wall stress