PXD024033 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targets of kinase/phosphatase signalling pair StkP/PhpP in S. pneumoniae and their involvement in response to cell wall stress |
Description | Streptococcus pneumoniae is an opportunistic human pathogen which encodes a single eukaryotic-type Ser/Thr protein kinase StkP and its functional counterpart protein phosphatase PhpP. These signalling enzymes play a crucial role in the coordination of cell division and growth in pneumococcus. In this study, we determined the proteomic and phosphoproteomic profiles of relevant mutants. A comparison with the wild type provided a representative data set of new phosphoacceptor sites and StkP-dependent substrates. StkP phosphorylates key proteins involved in cell division and cell wall biosynthesis in unencapsulated laboratory Rx1 strain as well as encapsulated virulent strain D39. Moreover, we show that StkP plays important role in eliciting an adaptive response induced by cell wall directed antibiotic. Phosphorylation of WalK sensor kinase and decline of the abundance of WalK and proteins of core WalR/K regulon implies cross-talk between StkP and WalR/K two-component system. Inspection of proteome profiles revealed gene clusters regulated by catabolite control mechanism suggesting a tight coupling of carbon flow and cell wall homeostasis. The disbalance of steady-state protein phosphorylation in the mutants as well as after antibiotic treatment is accompanied by accumulation of global Spx regulator indicating Spx-mediated envelope stress response. In summary, StkP translates the sensed signal about the cell-wall status to key cell division and regulatory proteins and in this way controls cell cycle and cell wall homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:51:34.379.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ivo Fabrik |
SpeciesList | scientific name: Streptococcus pneumoniae; NCBI TaxID: NCBITaxon:1313; scientific name: Streptococcus pneumoniae (strain ATCC BAA-255 / R6); NCBI TaxID: 171101; scientific name: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466); NCBI TaxID: 373153; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-07 23:56:32 | ID requested | |
1 | 2022-05-12 06:17:17 | announced | |
⏵ 2 | 2023-11-14 08:51:35 | announced | 2023-11-14: Updated project metadata. |
Publication List
Ulrych A, Fabrik I, Kup, č, í, k R, Vajrychov, á M, Doubravov, á L, Branny P, Cell Wall Stress Stimulates the Activity of the Protein Kinase StkP of Streptococcus pneumoniae, Leading to Multiple Phosphorylation. J Mol Biol, 433(24):167319(2021) [pubmed] |
Keyword List
submitter keyword: phosphatase PhpP, phosphoproteome, cell wall stress, Ser/Thr protein kinase StkP, signalling, ampicillin treatment,Streptococcus pneumoniae |
Contact List
Pavel Branny |
contact affiliation | Institute of Microbiology of the CAS, v. v. i., Videnska 1083, 142 20 Prague, Czech Republic |
contact email | branny@biomed.cas.cz |
lab head | |
Ivo Fabrik |
contact affiliation | Biomedical Research Center, University Hospital Hradec Kralove |
contact email | ivo.fabrik@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD024033
- Label: PRIDE project
- Name: Targets of kinase/phosphatase signalling pair StkP/PhpP in S. pneumoniae and their involvement in response to cell wall stress