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PXD023988-1

PXD023988 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePANNEXIN-1 CHANNEL REGULATES NUCLEAR CONTENT PACKAGING INTO APOPTOTIC BODIES AND THEIR SIZE
DescriptionApoptotic bodies (ApoBDs), which are large extracullular vesicles exclusively released by apoptotic cells, possess therapeutically exploitable properties including biomolecule loadability and transferability. However, current limited understanding of ApoBD biology has hindered its exploration for clinical use. Particularly, as ApoBD-accompanying cargoes (e.g. nucleic acids and proteins) have major influence on their functionality, further insights into the mechanism of biomolecule sorting into ApoBDs are critical to unleash their therapeutic potential. Previous studies suggested pannexin 1 (PANX1) channel, a negative regulator of ApoBD biogenesis, can modify synaptic vesicle contents. We also reported trovafloxacin (a PANX1 inhibitor) increasing proportion of ApoBDs containing DNA. Therefore, we sought to define the role of PANX1 in regulating the sorting of nuclear content into ApoBDs. Here, using flow cytometry and label-free quantitative proteomic analyses, we showed that targeting PANX1 activity during apoptosis, via either pharmacological inhibition or genetic disruption, resulted in enrichment of both DNA and nuclear proteins in ApoBDs that were unexpectedly smaller in size. Our data suggest that PANX1, besides being a key regulator of ApoBD formation, also functions as a negative regulator of nuclear content packaging and modulator of ApoBD size.
HostingRepositoryPRIDE
AnnounceDate2021-04-08
AnnouncementXMLSubmission_2021-04-07_17:58:23.846.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPamali Fonseka
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-04 01:22:00ID requested
12021-04-07 17:58:24announced
Publication List
Phan TK, Fonseka P, Tixeira R, Pathan M, Ang CS, Ozkocak DC, Mathivanan S, Poon IKH, PAnnexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size. Proteomics, ():e2000097(2021) [pubmed]
Keyword List
submitter keyword: Apoptotic bodies, extracellular vesicles, pannexin 1, cargo sorting, nuclear proteins, DNA
Contact List
Suresh Mathivanan
contact affiliationLa Trobe University, Department of Biochemistry and Genetics
contact emails.mathivanan@latrobe.edu.au
lab head
Pamali Fonseka
contact affiliationLa Trobe University
contact emailp.fonseka@latrobe.edu.au
dataset submitter
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Dataset FTP location
PRIDE project URI
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