PXD023988-1

PXD023988 is an original dataset announced via ProteomeXchange.

Title	PANNEXIN-1 CHANNEL REGULATES NUCLEAR CONTENT PACKAGING INTO APOPTOTIC BODIES AND THEIR SIZE
Description	Apoptotic bodies (ApoBDs), which are large extracullular vesicles exclusively released by apoptotic cells, possess therapeutically exploitable properties including biomolecule loadability and transferability. However, current limited understanding of ApoBD biology has hindered its exploration for clinical use. Particularly, as ApoBD-accompanying cargoes (e.g. nucleic acids and proteins) have major influence on their functionality, further insights into the mechanism of biomolecule sorting into ApoBDs are critical to unleash their therapeutic potential. Previous studies suggested pannexin 1 (PANX1) channel, a negative regulator of ApoBD biogenesis, can modify synaptic vesicle contents. We also reported trovafloxacin (a PANX1 inhibitor) increasing proportion of ApoBDs containing DNA. Therefore, we sought to define the role of PANX1 in regulating the sorting of nuclear content into ApoBDs. Here, using flow cytometry and label-free quantitative proteomic analyses, we showed that targeting PANX1 activity during apoptosis, via either pharmacological inhibition or genetic disruption, resulted in enrichment of both DNA and nuclear proteins in ApoBDs that were unexpectedly smaller in size. Our data suggest that PANX1, besides being a key regulator of ApoBD formation, also functions as a negative regulator of nuclear content packaging and modulator of ApoBD size.
HostingRepository	PRIDE
AnnounceDate	2021-04-08
AnnouncementXML	Submission_2021-04-07_17:58:23.846.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pamali Fonseka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-04 01:22:00	ID requested
⏵ 1	2021-04-07 17:58:24	announced

Phan TK, Fonseka P, Tixeira R, Pathan M, Ang CS, Ozkocak DC, Mathivanan S, Poon IKH, Pannexin-1 channel regulates nuclear content packaging into apoptotic bodies and their size. Proteomics, 21(13-14):e2000097(2021) [pubmed]

submitter keyword: Apoptotic bodies, extracellular vesicles, pannexin 1, cargo sorting, nuclear proteins, DNA

Suresh Mathivanan
contact affiliation	La Trobe University, Department of Biochemistry and Genetics
contact email	s.mathivanan@latrobe.edu.au
lab head
Pamali Fonseka
contact affiliation	La Trobe University
contact email	p.fonseka@latrobe.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023988
     1. Label: PRIDE project
     2. Name: PANNEXIN-1 CHANNEL REGULATES NUCLEAR CONTENT PACKAGING INTO APOPTOTIC BODIES AND THEIR SIZE