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PXD023951-1

PXD023951 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvaluation and Optimization of High-Field Asymmetric Waveform Ion Mobility Spectrometry for Multiplexed Quantitative Site-specific N-glycoproteomics
DescriptionThe heterogeneity and complexity of glycosylation hinder the depth of site-specific glycoproteomics analysis. High-field asymmetric-waveform ion-mobility spectrometry (FAIMS) has shown to improve the scope of bottom-up proteomics. The benefits of FAIMS for quantitative N-glycoproteomics have not been investigated yet. In this work, we optimized FAIMS settings for N-glycopeptide identification, with or without the tandem mass tag (TMT) label. The optimized FAIMS approach significantly increased the identification of site-specific N-glycopeptides derived from the purified IgM protein or human lymphoma cells. We explored in detail the changes in FAIMS mobility caused by N-glycopeptides with different characteristics, including TMT labeling, charge state, glycan type, peptide sequence, glycan size and precursor m/z. Importantly, FAIMS also improved multiplexed N-glycopeptide quantification, both with the standard MS2 acquisition method and with our recently developed Glyco-SPS-MS3 method. The combination of FAIMS and Glyco-SPS-MS3 provided the highest quantitative accuracy and precision. Our results demonstrate the advantages of FAIMS for improved mass-spectrometry-based qualitative and quantitative N-glycoproteomics.
HostingRepositoryPRIDE
AnnounceDate2021-05-05
AnnouncementXMLSubmission_2021-05-05_02:03:40.117.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPan Fang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-02 23:27:03ID requested
12021-05-05 02:03:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: FAIMS N-glycoproteomics
Contact List
Kuan-Ting Pan
contact affiliationmax planck institute for biophysical chemistry
contact emailkpan@mpibpc.mpg.de
lab head
Pan Fang
contact affiliationFudan University
contact emailpfang@mpibpc.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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