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PXD023936

PXD023936 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExploring the glycosylation state of the Cryptosporidium parvum CpTSP protein family-HILIC glycopeptide enrichment
DescriptionCryptosporidium parvum is a zoonotic apicomplexan parasite and a common cause of diarrheal disease worldwide. The development of vaccines to prevent or limit infection remains an important goal for tackling these diarrheal diseases, which are a significant cause of infant morbidity in the developing world. The only approved vaccine against an apicomplexan parasite targets conserved adhesins possessing a thrombospondin repeat (TSR) domains. Orthologous TSR domain-containing proteins are commonplace in the apicomplexa and C. parvum possess 12 such proteins. Here, we explore the molecular evolution and conservation of these proteins and examine their abundance in C. parvum oocysts to assess the likelihood that they may be useful as vaccine candidates. We go onto examine the glycosylation states of these proteins using antibody-enabled and ZIC-HILIC enrichment techniques, which revealed that these proteins are modified with C-linked Hex and N-linked Hex5-6HexNAc2 glycans.
HostingRepositoryPRIDE
AnnounceDate2023-01-12
AnnouncementXMLSubmission_2023-01-11_18:49:25.886.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNichollasScott
SpeciesList scientific name: Cryptosporidium parvum Iowa II; NCBI TaxID: 353152;
ModificationListcomplex glycosylation
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-02 01:42:59ID requested
12023-01-11 18:49:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cryptosporidium,Glycosylation, thrombospondin repeat
Contact List
NichollasScott
contact affiliationDepartment of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Parkville, Victoria 3010, Australia.
contact emailnichollas.scott@unimelb.edu.au
lab head
NichollasScott
contact affiliationUniversity of Melbourne
contact emailnichollas.scott@unimelb.edu.au
dataset submitter
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Dataset FTP location
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